The line-up announced by Jerzy Brzęczek on Monday includes two footballers who have never before played for the Polish national team.
The two fresh faces, defender Hubert Matynia and striker Adam Buksa, are both from Polish top-flight side Pogoń Szczecin.
Poland will play a friendly against the Czech Republic in the northern Polish city of Gdańsk on November 15.
Five days later the Poles will take on Portugal in their fourth and last match in the group stage of the UEFA Nations League, a new competitive football format in Europe.
Poland in mid-October lost 0-1 to Italy in their third match of the Nations League and faced relegation to a lower tier of the new football format.
Poland last month lost 2-3 to Portugal in their second match of the Nations League, a sobering home defeat that exposed the side's frailties and left fans in low spirits.
The Poles in early September drew 1-1 with Italy in their first match in the group stage of the Nations League.
That game, in Bologna, was a promising debut by Jerzy Brzęczek as manager of the Polish national squad. He took over after Poland’s early exit from the World Cup in Russia.
Here is the full Poland squad for the November matches against the Czech Republic and Portugal:
Goalkeepers: Łukasz Fabiański (West Ham United), Łukasz Skorupski (Bologna FC), Wojciech Szczęsny (Juventus).
Defenders: Jan Bednarek (Southampton), Bartosz Bereszyński (Sampdoria), Kamil Glik (AS Monaco), Artur Jędrzejczyk (Legia Warsaw), Marcin Kamiński (Fortuna Duesseldorf), Tomasz Kędziora (Dynamo Kyiv), Hubert Matynia (Pogoń Szczecin), Paweł Olkowski (Bolton Wanderers), Rafał Pietrzak (Wisła Kraków).
Midfielders: Jakub Błaszczykowski (VfL Wolfsburg), Przemysław Frankowski (Jagiellonia Białystok), Jacek Góralski (Ludogorets Razgrad), Kamil Grosicki (Hull City), Damian Kądzior (Dinamo Zagreb), Mateusz Klich (Leeds United), Grzegorz Krychowiak (Lokomotiv Moscow), Damian Szymański (Wisła Płock), Piotr Zieliński (Napoli).
Forwards: Adam Buksa (Pogoń Szczecin), Robert Lewandowski (Bayern Munich), Arkadiusz Milik (Napoli), Krzysztof Piątek (Genoa CFC).